Structural control of cytochrome P450-catalyzed ω-hydroxylation
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چکیده
منابع مشابه
Short Communication Specificity of Cytochrome P450 2A3-Catalyzed a-Hydroxylation of N*-Nitrosonornicotine Enantiomers
N*-nitrosonornicotine (NNN) induces tumors in the rat nasal cavity and esophagus and is believed to be a causative agent for esophageal cancer in tobacco users. To exert its carcinogenic potential, NNN must be metabolically activated by a-hydroxylation at either the 2*or 5*-carbon. We previously reported that the human cytochrome P450 (P450), 2A6, efficiently and specifically catalyzed NNN 5*-h...
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The title probes are precursors to kinetically calibrated, aryl-substituted cyclopropylcarbinyl radicals that rearrange with picosecond lifetimes. Applications in studies of cytochrome P450-catalyzed hydroxylation reactions are reviewed. Initially confusing results regarding lifetimes of radicals in the hydroxylation reactions were resolved when second-generation probes that distinguish between...
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Cytochrome P450 3A4 is known to catalyze the metabolism of both endogenous substrates (such as the 6b-hydroxylation of testosterone) and many important therapeutic agents, including the Ndemethylation of erythromycin. However, erythromycin and testosterone have been reported to have little or no effect on the metabolism of each other by recombinant CYP3A4. In an effort to understand the basis o...
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Cytochrome P450 3A4 is known to catalyze the metabolism of both endogenous substrates (such as the 6 beta-hydroxylation of testosterone) and many important therapeutic agents, including the N-demethylation of erythromycin. However, erythromycin and testosterone have been reported to have little or no effect on the metabolism of each other by recombinant CYP3A4. In an effort to understand the ba...
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Phenytoin, 5,5-diphenylhydantoin, is a widely used anticonvulsant agent with a variety of toxicities, including drug interactions. The formation of four oxidative metabolites, 4'-hydroxylated (4'-HPPH), 3'-hydroxylated (3'-HPPH), a catechol (3',4'-diHPPH), and the 3',4'-dihydrodiol form of phenytoin was examined in rat liver microsomes. In 11 cDNA-expressed rat P450 enzymes tested, CYP2C6 had t...
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ژورنال
عنوان ژورنال: Archives of Biochemistry and Biophysics
سال: 2011
ISSN: 0003-9861
DOI: 10.1016/j.abb.2010.08.011